Invenra is seeking an experienced, instrumentation-savvy analytical Scientist to join our Protein Characterization/Analytics team. This individual will report to the Director of the Analytics group and will assume responsibility for protein chromatography quality control, assay development, and instrumentation troubleshooting (including maintenance/repair) to support Invenra’s numerous pre-clinical protein biotherapeutic programs. Key aspects of the job entail (1) analyzing protein samples by HPLC, (2) polishing protein samples by HPLC/FPLC, (3) ensuring HPLC and FPLC instrumentation are within operational specifications, (4) monitoring and addressing data quality issues generated by HPLC/FPLC, (5) leading routine maintenance and repair of chromatographic instruments, (6) developing new assays to streamline purification/characterization to expand our assay portfolio, and (7) interpretation data to support various research projects and product quality evaluations.
The position requires high analytical acumen with well-honed scientific and problem-solving skills suited for a pre-clinical analytical lab. We seek a candidate with strong technical competencies regarding operation and repair of chromatographic instrumentation and expert grasp of the fundamentals of chromatographic separations including underlying chemistry. Experience analyzing/purifying biomolecules including mono-/multi-specific antibodies, nanobodies (VHH), cell-surface proteins, and affinity-tagged proteins is highly desired. Experience using and interpreting data from multiangle light scattering instrumentation (MALS) is a plus. The ideal candidate must quickly interpret analytical results and make well-reasoned decisions regarding quality and work to resolve quality issues and escalate concerns to the Director and appropriate scientific teams when required.
Ideal candidates will be an innovative thinker with the ability to work independently, design and carry out experiments and propose creative solutions to challenging problems and thus must bring intellectual, professional and personal values that complement the founding team: the highest standard of integrity, a tremendous work ethic and the passion to succeed.
- QA/QC characterization of antibodies, antigens, and other engineered proteins by HPLC using SEC, HIC, IEX, and SMAC techniques with UV, fluorescence, and/or MALS detection. Must endure data quality meets or exceeds quality criteria.
- Affinity purifying and/or 2nd-step polishing proteins by gravity filtration, HPLC, and/or FPLC.
- Employing new strategies for protein characterization using chromatographic techniques.
- Developing and/or modifying purification techniques to improve protein yield, protein purity, and sample throughput.
- Establishing suitable standards and controls for new assays and writing standard operating procedures for use.
- Preparing analytical standards, chromatographic mobile phases, and reagent stocks.
- Analyzing and interpreting data from multiple assays, compiling and communicating results.
- Serving as a representative from Analytics on project core teams.
Knowledge, Skills, and Abilities:
- Mastery of chromatographic separations and operation of analytical instrumentation is required. Experience with protein chromatography strongly preferred.
- Must be capable of routine maintenance of HPLC/FPLC instrumentation including replacing pump seals/pistons, injection valve repair, and replacement of sampling needles and in-line filters. Preference will be shown for candidates with experience with Agilent 1100/1200 HPLC systems and GE (Cytiva) AKTA FPLC instruments.
- The ideal candidate should be capable of advanced HPLC/FPLC instrument troubleshooting and repair including diagnosing faulty electronic boards and performing major hardware repairs (replacing pump assemblies, replacing transport carriages, rebuilding detection cells and degassers, etc.).
- Must have solid grasp of underlying chemistry governing chromatographic separations including the variables that can be adjusted to achieve the desired experimental outcome.
- The ability to execute routine work at expected quality with minimal supervisory oversight is a requirement.
- Must be willing to mentor junior scientists, research associates, and interns while fostering a laboratory environment comprising analytical rigor, creativity, and accountability.
- Must adhere to standard operating procedures when required.
- Must be reliable, have a professional attitude, and maintain positive group dynamic through effective and timely communication.
- Should embrace working in a fast-paced environment, with multiple projects and evolving priorities.
Education and Experience:
The target candidate at the Scientist level will have a Ph.D. in chemistry, biochemistry, or other biological/physical science discipline (molecular and cell biology, immunology, physics etc.) with 0-2 years of industry experience. Newly minted Ph.D.s are encouraged to apply. Experience and technical aptitude will be considered as a substitute for formal education for candidates with a bachelor’s or master’s degree with 5+ or 3+ years of experience, respectively, relevant to the requirements/preferences for this position.